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By inhibiting NNMT, 5-Amino-1MQ increases cellular SAM and NAD+ levels, enhances NAD+ salvage pathway flux, activates SIRT-1, boosts oxidative phosphorylation (OXPHOS), and influences cellular energy homeostasis and epigenetic modifications

Similar results were demonstrated in vitro with C2C12 cells that closely mimic muSCs, where NNMTi treatment promoted myoblast differentiation and supported metabolic changes in NAD+ salvage pathway-related cellular metabolites[5]

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