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Gene silencing was verified by detecting proteins via immunoblotting analysis after transient transfection with siRNA

In recent years, small molecule antagonists, antisense oligonucleotides, and monoclonal antibodies targeting GCGR have been developed and demonstrated efficacy in improving glycemic control in both rodent models of diabetes and human patients with diabetes 8,9,10,11,12,13

Similarly, examining how acute and/or repeated applications of neuropeptides affect expression of mucins (e.g., muc5AC, muc5B) in asthma, COPD, and CF would reveal a potentially causative role for neuropeptides in airway mucus phenotypes

Detailed bacteriostatic water ratios and techniques apply across all reconstituted peptides

While freezing itself causes no damage, the condensation during thawing can introduce moisture

Sasaki M, Karube A, Karube Y, Watari M, Sakuragi N, Fujimoto S, Dahiya R (2005) GGC and StuI polymorphism on the androgen receptor gene in endometrial cancer patients