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Supplies Needed Quantities below assume a 48 week course of daily injections at a moderate dose
The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm
per each mL: Thiamine HCl 100 mg, Dexpanthenol 2 mg, Riboflavin 5' -Phosphate 2 mg, Pyridoxine HCl 2 mg, Niacinamide 100 mg Vitamin B-Complex 100 Injection is a sterile solution containing essential B vitamins for intramuscular or slow intravenous administration
For each assay, cells were plated in duplicate, with one plate containing CellTox Green Dye added according to the Express, No-Step Addition at Seeding protocol described in the CellTox Green Cytotoxicity Assay Technical Manual #TM375 (10l of CellTox Green Dye per 5ml of cells)