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GLUT1 is palmitoylated at Cys207 and S-palmitoylation is required for maintaining GLUT1 PM localization To determine the site of GLUT1 palmitoylation, we mutated every cysteine to serine in the GLUT1 protein, and their S-palmitoylation levels were characterized by using the alkynyl palmitic acid incorporation assay

Animals acclimated to the animal room for 3 days should be brought into the laboratory

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