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Afterward, 40 L of PL solution were pre-incubated for 15 min at 37 C with 40 L of varying concentrations (0.2510 mg/mL) of each extract, then 4-nitrophenyl butyrate ( p -NPB) substrate at a concentration of 10 mM in pure ethanol was added

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Phenibut HCL is highly water-soluble (~200 mg/mL) and suited to aqueous solution preparation