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The extracted RNA was then reverse-transcribed into cDNA using the PrimeScript RT reagent kit (RR037A, Takara Biotechnology, Osaka, Japan)

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Nunc T-25 EasYFLask tissue culture flasks (ThermoFisher Catalog #156,340, Waltham, MA) were coated for 2 h with Basal Medium (BEBM medium), collagen, bovine serum albumin, and fibronectin prior to subculturing

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