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The results of an experiment conducted on murine Th1 and Th2 cells suggest that NO can exacerbate the inflammatory response in asthma by disrupting the balance between T helper cells (100)

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In enzymes, tyrosine residues are often associated with active sites, alteration of which can change enzyme specificity or wipe out activity entirely

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It is also possible that while heating is effective at disrupting protein shapes and eliminating enzyme activity, it does not eliminate ice nucleation activity as the proteins are twisted into new shapes that have ice nucleation sites, similar to the hypothesis that defects and aggregation of large proteins are a source of ice nucleation sites 55

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