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To identify regulatory T cells, cells were fixed and permeabilized using the FoxP3 Staining Buffer Set (eBioscience, San Diego, CA)

2.3 Prenatal ETS exposure measurement The researchers asked the mothers the following question to measure their prenatal ETS exposure throughout pregnancy: Did anyone in the family living with the mother smoke (including e-cigarettes) during pregnancy? (Two answer options: No or Yes.) If the answer was yes, then prenatal ETS exposure during the pregnancy was considered to be present

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Maternal undernutrition influences placental-fetal development