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Needle Insertion: Hold the syringe like a pencil and insert the needle quickly and smoothly at a 90-degree angle into the skin

10.1038/nrg1047 143

Absorbance was detected by using a microplate spectrophotometer (Bio-Rad) at 490 nm

Although the method through which lobeline disrupts SG disassembly is unknown, the act of disrupting canonical stress pathway dynamics and preventing timely stress recovery is the important factor