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10.1152/ajpheart.00206.2015 127 MargaritelisN

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and lactatate dehydrogenase, LDH) were measured using an auto-analyzer AU-800 (Olympus, Tokyo), and troponin T was assessed by a rapid quantitative assay using the Cardiac Reader (Roche Diagnostics, Mannheim, Germany)

The 28-day guideline is based on pharmaceutical stability studies that determine the length of time the preservative can reliably maintain sterility through multiple punctures