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The frequency: For maintenance (for example, preventing LPP flares), dosing may be as infrequent as once every few months

For medical issues and problems: seek medical advice

doi: 10.1007/s00726-003-0026-8 132

Cancer promotion concerns arise from IGF-1's well-established role in cellular proliferation, survival, and growth

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Cell pellets were carefully thawed on ice and cells resuspended in 7 mL PBS supplemented with 4 L NP-40 (Thermo Scientific) and 100 L cOmplete protease inhibitor cocktail (Sigma-Aldrich), 1 L DNase I (10 mg/mL), lysozyme (Roth), and 800 L glycerol (Roth)